2. Signaling Pathways
  3. Cell Cycle/DNA Damage
  4. DNA/RNA Synthesis

DNA/RNA Synthesis

DNA/RNA Synthesis

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RNA synthesis, which is also called DNA transcription, is a highly selective process. Transcription by RNA polymerase II extends beyond RNA synthesis, towards a more active role in mRNA maturation, surveillance and export to the cytoplasm.

Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template, with DNA ligase creating the final phosphodiester bond to fully repair the DNA.DNA ligases discriminate against substrates containing RNA strands or mismatched base pairs at positions near the ends of the nickedDNA. Bleomycin (BLM) exerts its genotoxicity by generating free radicals, whichattack C-4′ in the deoxyribose backbone of DNA, leading to opening of the ribose ring and strand breakage; it is an S-independentradiomimetic agent that causes double-strand breaks in DNA.

First strand cDNA is synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. The remaining overhangs are converted into blunt ends using exonuclease/polymerase activity. After adenylation of the 3′ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare the samples for hybridization. Cell cycle and DNA replication are the top two pathways regulated by BET bromodomain inhibition. Cycloheximide blocks the translation of mRNA to protein.

DNA/RNA Synthesis Isoform Specific Products:

DNA/RNA Synthesis Isoform Comparison
Cat. No. Product Name Effect Purity Chemical Structure
  • HY-69014
    2-O-Methylcytosine
    		99.97%
    2-O-Methylcytosine, an O-alkylated analogue a DNA adduct, is the damaged nucleobase.
    2-O-Methylcytosine
  • HY-174346
    Skp2-IN-4
    		Inhibitor 98.60%
    Skp2-IN-4 is an Skp2 inhibitor with a IC50 of 0.38  μM for Skp2-Cks1 binding. Skp2-IN-4 improves anti-tumor activity, inhibits the proliferation and induces S phase arrest by targeting Skp2. Skp2-IN-4 significantly enhances Cisplatin (HY-17394) chemosensitivity by suppressing the tumor cell stemness in NCl-H1299 xenograft mice model, promising for lung cancer and esophageal cancer research.
    Skp2-IN-4
  • HY-121106
    9-Deazaguanine
    		99.01%
    9-Deazaguanine is a nucleoside analog with potent inhibitory activity against purine nucleoside phosphorylase (PNP).
    9-Deazaguanine
  • HY-P3187
    exo-β-1,4-Xylosidase, Cellulosimicrobium cellulans sp. 21
    Exo-1,4-β-xylosidase is an exonuclease that specifically acts on the β-1,4 glycosidic bonds at the non-reducing ends of xylan and xylooligosaccharides. Exo-1,4-β-xylosidase is Ca2+-dependent and reversibly binds to metal ions to catalyze the hydrolysis of β-1,4 glycosidic bonds, thereby degrading xylan to produce xylose. Exo-1,4-β-xylosidase can be used in research fields such as lignocellulose bioconversion, bioethanol production, and optimization of xylan saccharification processes.
    exo-β-1,4-Xylosidase, Cellulosimicrobium cellulans sp. 21
  • HY-119993
    AR03
    		Inhibitor 99.1%
    AR03 (BMH-23) is an apurinic/apyrimidinic endonuclease 1 (Ape1) inhibitor with an IC50 of 2.1 µM. AR03 has low affinity for double-stranded DNA. AR03 potentiates the cytotoxicity of methyl methanesulfonate and temozolomide in SF767 cells.
    AR03
  • HY-121713
    Aniline mustard
    		Inhibitor 99.72%
    Aniline mustard (N, n-BIS (2-chloroethyl) aniline) is an anticancer agent.
    Aniline mustard
  • HY-135218A
    AV-153 free base
    		Activator 99.59%
    AV-153 free base, a 1,4-dihydropyridine (1,4-DHP) derivative, is an antimutagenic. AV-153 free base intercalates to DNA in a single strand break and reduces DNA damage, stimulates DNA repair in human cells in vitro. AV-153 free base interacts with thymine and cytosine and has an influence on poly(ADP)ribosylation. AV-153 free base has anti-cancer activity.
    AV-153 free base
  • HY-151986
    FOXM1-IN-1
    		Inhibitor 99.86%
    FOXM1-IN-1 is a potent FOXM1 inhibitor with an IC50 value of 2.65 µM. FOXM1-IN-1 shows antiproliferative activity. FOXM1-IN-1 decreases the the expression of FOXM1, PLK1, CDC25B protein.
    FOXM1-IN-1
  • HY-156581
    WRN inhibitor 5
    		Inhibitor 98.12%
    WRN inhibitor 5, a cyclic vinyl sulfone compound, is a Wemer Syndrome ATP dependent helicase enzyme (WRN) inhibitor. WRN inhibitor 5 can be used for the research of cancer, including cancers characterized by microsatellite instability (MSI) and/or defective DNA mismatch repair system (dMMR).
    WRN inhibitor 5
  • HY-N1457
    Chrysosplenetin
    		Inhibitor 99.84%
    Chrysosplenetin is an orally active polymethoxyflavone. Chrysosplenetin exerts anticancer effects by inhibiting topoisomerase, protecting DNA and inducing apoptosis. Chrysosplenetin acts as an antimalarial sensitizer, reverses Artemisinin (HY-B0094) resistance by inhibiting and downregulating P-glycoprotein, and enhances the efficacy of Artemisinin. Chrysosplenetin promotes bone formation by activating the Wnt/β-catenin pathway and exerts anti-osteoporotic effects.
    Chrysosplenetin
  • HY-W008598
    2-Bromo-4'-fluoroacetophenone
    		Inhibitor 99.15%
    2-Bromo-4'-fluoroacetophenone (p-Fluorophenacyl bromide) is a photoinducible DNA cleavage inducer.2-Bromo-4'-fluoroacetophenone generates fluorine-substituted phenyl radicals upon excitation and irradiation to cleave DNA, converting circular supercoiled DNA to circular relaxed and linear DNA forms.2-Bromo-4'-fluoroacetophenone exhibits concentration-dependent DNA cleaving activity.2-Bromo-4'-fluoroacetophenone can be used for the research of cancer.
    2-Bromo-4'-fluoroacetophenone
  • HY-W116007
    Octamethylcyclotetrasiloxane
    		Inhibitor 99.86%
    Octamethylcyclotetrasiloxane promotes the anchorage-independent growth of MCF-10A and MCF-10F cells. Octamethylcyclotetrasiloxane induces DNA damage, inhibits the expression of DNA-repairing protein BRCA1 under long-term and high-concentration exposure. Octamethylcyclotetrasiloxane exhibits intrinsic estrogenic activity.
    Octamethylcyclotetrasiloxane
  • HY-W093086
    DMT-2'-F-Bz-dA
    		99.58%
    DMT-2'-F-Bz-dA is a phosphoramidite monomer that can be used for oligonucleotide synthesis.
    DMT-2'-F-Bz-dA
  • HY-158772
    m7G(5')ppp(5')(2'OMeA)pU ammonium solution (100 mM)
    		99.6%
    m7G(5')ppp(5')(2'OMeA)pU ammonium is a cap analog that can be used for in vitro transcription of mRNA.
    m7G(5')ppp(5')(2'OMeA)pU ammonium solution (100 mM)
  • HY-158664
    2-Amino-ATP sodium solution (100 mM)
    		99.92%
    2-Amino-ATP sodium solution (100 mM) is a labeled modified deoxyoligonucleotide (dNTP) that can release pyrophosphate to produce fluorescence and has special applications in gene synthesis and sequencing.
    2-Amino-ATP sodium solution (100 mM)
  • HY-173189B
    2-5A TEA
    		Inhibitor
    2-5A (2′,5′-ApApA; 2′,5′-trioligoadenylate; 5'-O-Triphosphoryladenylyl-(2'→5')-adenylyl-(2'→5')-adenosine) TEA is a 5'-triphosphorylated (2',5') oligoadenylate. 2-5A TEA is an immunotransmitter that fuels RNase L immunity. 2-5A TEA degrades viral mRNA and inhibits protein synthesis by binding to RNase L and activating its endoribonuclease activity. 2-5A TEA can be used in RSV and cancer research.
    2-5A TEA
  • HY-21648
    2'-OMe-Ac-C Phosphoramidite
    		98.0%
    2'-OMe-Ac-C Phosphoramidite is a modified phosphoramidite and can be used for the oligonucleotide synthesis. 2'-OMe-Ac-C Phosphoramidite contributes to improving the stability and activity of oligonucleotides.
    2'-OMe-Ac-C Phosphoramidite
  • HY-W039722
    5-Fluorocytidine
    		Inhibitor 99.88%
    5-Fluorocytidine is a member of cytidines, inhibits maturation of the 45S ribosomal RNA precursor.
    5-Fluorocytidine
  • HY-W012311
    3′-Deoxythymidine
    		Inhibitor 99.13%
    3′-Deoxythymidine (2′,3′-Dideoxythymidine), a nucleoside analog, inhibits eukaryotic cellular and viral DNA polymerases, and inhibits retrovirus infection in some cells.
    3′-Deoxythymidine
  • HY-18572S2
    2,4-D-d5
    		Inhibitor 99.39%
    2,4-D-d5 (2,4-Dichlorophenoxyacetic acid-d5) is the deuterium labeled 2,4-D (HY-18572). 2,4-D (2,4-Dichlorophenoxyacetic acid) is a selective systemic herbicide for the control of broad-leaved weeds. 2,4-D acts as a plant hormone, causing uncontrolled growth in the meristematic tissues. 2,4-D inhibits DNA and protein synthesis and thereby prevents normal plant growth and development.
    2,4-D-d<sub>5</sub>
Cat. No. Product Name / Synonyms Application Reactivity
DNA/RNA Synthesis Isoform Comparison

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